ARCHIVES OF CLINICAL MICROBIOLOGY: MULTI DRUG RESISTANCE PATTERNS OF SHIGA TOXIN– PRODUCING ESCHERICHIA COLI (STEC) ANDNON – STEC ISOLATES FROM MEATS, RTE MEATFOODS, DRINKING WATER AND HUMAN DIARRHOEICSAMPLES OF PUNJAB, INDIA


Autor: VV. AA.
Fecha: 2012
Género: Ciencias
Editorial: IMEDPUB
Páginas
ISBN: cdlcm19898421
Idioma: VV. AA.


Antimicrobial susceptibility testing was performed on a total of 253 [203 shiga toxin-producing Escherichia coli (STEC) and 50 non-STEC]Escherichia coli isolates from difer-ent raw meats, RTE meat foods, drinking water and human diarrhoeic samples in Pun-jab, India. Among the 20 antimicrobial agents tested, resistance was most frequentfor penicillin (253/253, 100%) followed by linezolid (249/253, 98.4%), erythromycin(245/253, 96.8%), streptomycin (232/253, 91.7%), tetracycline (223/253, 88.1%), am-picillin 123/253,48.2%), cephotaxime (106/253,41.9%), trimethoprim (93/253, 36.8%)),co-trimoxazole (85/253, 33.6%), cefaclor (84/253, 33.2%), amoxycillin (79/253, 31.3%),ciprofoxacin (78/253, 30.8%), kanamycin (76/253, 30.0%), norfoxacin (62/253, 24.5%),ofoxacin (60/253, 23.7%), chloramphenicol (35/253, 13.8%), Polymyxin-B (27/253,10.6%), colistin (25/253,9.9%), amikacin (22/253, 8.7%) and gentamycin (17/253, 6.7%).Out of 203 STEC isolates 73 (35.9%) showed resistance to more than 50% of the anti-biotics tested. Only one isolate from pork showed resistance to 90% of the antibioticstested. Cluster analysis also revealed that human isolates were diferent from otherE. coli isolated from meat and meat products sources. The distribution of resistancedeterminants for tetracycline and streptomycin was assessed by PCR in resistant iso-lates. The most common resistance determinants were tetA (60%) and tetB (27%).Forty seven per cent of the isolates contained both strA and strB genes, 33% and 10%isolates carried strA and strB genes, respectively and 10% of isolates did carry neitherstrA nor strB. Cloning and sequencing of tetA and tetB genes of O69 shiga toxin-pro-ducing E. coli isolate from bufalo meat showed 99-100% homology with publishedsequences of related isolates in GenBank. Antibiotic susceptibility studies revealedthat meat, RTE meat products, drinking water and human diarrhoeic samples fromPunjab, India contains multiple drug resistant strains of E. coli which may serve as areservoir for antibiotic resistance genes in the food environment and may transmitto humans through food chain.




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